Electrophoresis of Lipid-free Blood Serum

The finding of lipid substances in all electrophoretically separable serum proteins, in the case of w and ,&globulins in remarkably large proportions (Blix, Tiselius, and Svensson, 1941), raised the question of the possible r&e of the lipids in the electrophoretic behavior of these proteins. Experiments with sera from which the lipids had been largely removed, according to the mild extraction principle introduced by Hardy and Gardiner (1910), seemed likely to give certain information in this respect. Such experiments were accordingly carried out. At the same time some questions pertaining to the transport function of the serum proteins were taken into consideration and experimentally investigated.